Anti-Hemolytic Efficacy of Nano Emulsion Biologically Produced from (Cumin) Essential Oil

The study presented by student Suhad Majed Atwan from Department of Life Sciences succeeded in producing a (nano emulsion) from biological sources that have effective antioxidant activity and are biocompatible with the human body which are present in its food chain, where botany and nanotechnology were combined. The nano emulsion was prepared from cumin seeds in several ways and was detected through an ultrasonic nanomaterial dispersion device and through a dynamic light scattering device (DLS).

The results of the detection indicated that the size of the nano emulsion droplets appeared with an average size of (12.5) nanometers and the value of poly dispersive index (PDI) appeared (0.3218), and the nano emulsion droplets appeared through the scanning electron microscope (SEM) in a semi-spherical shape. The active compounds of cumin oil and the nano emulsion were also investigated by gas chromatography-mass spectrometry (GC-MS) analysis, and the highest peak of the cumin aldehyde compound appeared in addition to other compounds.

The antioxidant activity was also studied through DPPH assay to determine the antioxidant activity of nano emulsions, which showed their effectiveness in inhibiting free radicals. In order to study the biocompatibility of nano emulsions through their interaction with blood, the ability of these nano emulsions to dissolve blood or not was also studied through visual examination as well as optical density study, and it was found that no dissolution of red blood cells occurred. The thesis was approved with a very good high grade.